Elucidating protein

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The two primary methods used for the ionization of protein in mass spectrometry are electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI).

Mass spectrometry is an important method for the accurate mass determination and characterization of proteins, and a variety of methods and instrumentations have been developed for its many uses.

In MALDI, the proteins are embedded within a matrix normally in a solid form, and ions are created by pulses of laser light.

Electrospray produces more multiply-charged ions than MALDI, allowing for measurement of high mass protein and better fragmentation for identification, while MALDI is fast and less likely to be affected by contaminants, buffers and additives.

In general, the protein are analyzed either in a "top-down" approach in which proteins are analyzed intact, or a "bottom-up" approach in which protein are first digested into fragments.

An intermediate "middle-down" approach in which larger peptide fragments are analyzed may also sometimes be used.

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